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Table 3 Performance of the real-time RT-RPA assay or LFS RT-RPA assay in comparison with the real-time RT-qPCR assay for detecting PPRV on clinical samples

From: Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus

  Real-time qPCR Performance characteristics (%)
Positive Negative Sensitivity Specificity
Real-time RPA Positive 18 0 90% 100%
Negative 2 17   
Total (n = 37) 20 17   
RPA LFD Positive 18 0 90% 100%
Negative 2 17   
Total (n = 37) 20 17   
  1. Samples include thirty-two samples collected from suspected cases of the sheep, and five serum samples collected from healthy sheep
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