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Table 3 Performance of the real-time RT-RPA assay or LFS RT-RPA assay in comparison with the real-time RT-qPCR assay for detecting PPRV on clinical samples

From: Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus

 

Real-time qPCR

Performance characteristics (%)

Positive

Negative

Sensitivity

Specificity

Real-time RPA

Positive

18

0

90%

100%

Negative

2

17

  

Total (n = 37)

20

17

  

RPA LFD

Positive

18

0

90%

100%

Negative

2

17

  

Total (n = 37)

20

17

  
  1. Samples include thirty-two samples collected from suspected cases of the sheep, and five serum samples collected from healthy sheep