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Table 2 Comparison of PPRV real-time RT-RPA assay and PPRV LFS RT-RPA assay with RT-qPCR assay on virus species, spiked samples, field samples and healthy sheep samples

From: Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus

Sample type Sample name real-time RT-RPA(min) LFS RT-RPA) RT-qPCR (Cq specific RT-qPCR(Cq)
Virus species PPRV Nigeria 75/1 4.6 + 18 18
GPV AV40 - - - 17
SPV Gulang2009 - - - 19
ORFV/Vaccine/CHA - - - 20
ORFV/HB/CHA - - - 22
FMDV/O/CHA - - - 21
FMDV/A/CHA - - - 19
FMDV/Asia1/CHA - - - 20
Spiked samples Liver 1 4.6 + 16 -
Liver 2 7.6 + 28 -
Liver 3 6 + 20 -
Lungs 1 4.3 + 15  
Lungs 2 5.3 + 17 -
Lungs 3 6 + 21 -
Stomach 1 6 + 23 -
Stomach 2 4 + 16 -
Stomach 3 5.3 + 18 -
Kidney 1 5.6 + 21 -
Kidney 2 6 + 24 -
Kidney 3 3.6 + 14 -
Lymphatic nodes 1 5.3 + 17 -
Lymphatic nodes 2 6 + 21 -
Lymphatic nodes 3 4 + 18 -
Skin 1 5.6 + 22 -
Skin 2 6 + 25 -
Skin 3 7.6 + 27 -
Field samples Lung - - - -
Spleen - - - -
Nasal swab - - - -
Kidney - - - -
Spleen 6.3 + 25 -
Nasal swab - - - -
Nasal swab -   - -
Kidney - - 31 -
lymphatic nodes - - - -
Nasal swab 6 + 23 -
Kidney - - - -
Spleen 4 + 19 -
Nasal swab - - - -
Lung 5 + 19 -
Spleen - - - -
Kidney - - 33 -
Spleen - - - -
Lung - - - -
Kidney 5 + 19 -
Nasal swab 7 + 24 -
Spleen 6.3 + 20 -
Lung 6.6 + 22 -
Spleen 5.3 + 20 -
Kidney 6 + 20 -
Spleen 5 + 18 -
Nasal swab 7.3 + 26 -
Kidney 7.6 + 27 -
Spleen 5.6 + 21 -
Lung 6.3 + 22 -
Lymphatic nodes 7.3 + 27 -
Kidney 6.6 + 24 -
Spleen 6 + 23 -
Healthy sheep samples Liver - - - -
Lungs - - - -
Stomach - - - -
Kidney - - - -
Skin - - - -
  1. Samples include PPRV、sheep and goat pox viruses 、foot-and-mouth disease virus and Orf virus, eighteen spiked samples, eighteen filed samples and five healthy sheep samples. CaPV capripoxvirus, GPV goat pox viruses, SPV sheep pox viruses, CHA China. specific qPCR specific qPCR tested the respective viruses used in this study, + : positive; - : negative
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