Table 2 Comparison of PPRV real-time RT-RPA assay and PPRV LFS RT-RPA assay with RT-qPCR assay on virus species, spiked samples, field samples and healthy sheep samples

Virus species

PPRV Nigeria 75/1

4.6

+

18

18

GPV AV40

-

-

-

17

SPV Gulang2009

-

-

-

19

ORFV/Vaccine/CHA

-

-

-

20

ORFV/HB/CHA

-

-

-

22

FMDV/O/CHA

-

-

-

21

FMDV/A/CHA

-

-

-

19

FMDV/Asia1/CHA

-

-

-

20

Spiked samples

Liver 1

4.6

+

16

-

Liver 2

7.6

+

28

-

Liver 3

6

+

20

-

Lungs 1

4.3

+

15

Lungs 2

5.3

+

17

-

Lungs 3

6

+

21

-

Stomach 1

6

+

23

-

Stomach 2

4

+

16

-

Stomach 3

5.3

+

18

-

Kidney 1

5.6

+

21

-

Kidney 2

6

+

24

-

Kidney 3

3.6

+

14

-

Lymphatic nodes 1

5.3

+

17

-

Lymphatic nodes 2

6

+

21

-

Lymphatic nodes 3

4

+

18

-

Skin 1

5.6

+

22

-

Skin 2

6

+

25

-

Skin 3

7.6

+

27

-

Field samples

Lung

-

-

-

-

Spleen

-

-

-

-

Nasal swab

-

-

-

-

Kidney

-

-

-

-

Spleen

6.3

+

25

-

Nasal swab

-

-

-

-

Nasal swab

-

-

-

Kidney

-

-

31

-

lymphatic nodes

-

-

-

-

Nasal swab

6

+

23

-

Kidney

-

-

-

-

Spleen

4

+

19

-

Nasal swab

-

-

-

-

Lung

5

+

19

-

Spleen

-

-

-

-

Kidney

-

-

33

-

Spleen

-

-

-

-

Lung

-

-

-

-

Kidney

5

+

19

-

Nasal swab

7

+

24

-

Spleen

6.3

+

20

-

Lung

6.6

+

22

-

Spleen

5.3

+

20

-

Kidney

6

+

20

-

Spleen

5

+

18

-

Nasal swab

7.3

+

26

-

Kidney

7.6

+

27

-

Spleen

5.6

+

21

-

Lung

6.3

+

22

-

Lymphatic nodes

7.3

+

27

-

Kidney

6.6

+

24

-

Spleen

6

+

23

-

Healthy sheep samples

Liver

-

-

-

-

Lungs

-

-

-

-

Stomach

-

-

-

-

Kidney

-

-

-

-

Skin

-

-

-

-