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Fig. 3 | Virology Journal

Fig. 3

From: Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus

Fig. 3

Determination of reaction temperature and time. a The LFS RT-RPA assay were performed respectively at different temperatures as they are shown in the figure. b After 10 min of isothermal amplification reaction, the slight test line is visible on the test strip, and when the amplification time is longer than 15 min or 20 min, the clear test line is visible on the test strip. Including the incubation time of 2 min, the whole assay time of the LFS RT-RPA assay is less than 25 min, and the results showed that this assay works effectively in a broad range of temperatures from 37 to 45 °C

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