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Table 2 Viral Plaque Assay and RT-PCR results in solid tissues from marmosets exposed to aerosolized EEEV

From: Characterization and pathogenesis of aerosolized eastern equine encephalitis in the common marmoset (Callithrix jacchus)

 

Animal ID (Inhaled Dose, PFU)

Tissue

marmoset #1 (2.4 × 101)

marmoset #2 (1.15 × 103)

marmoset #3 (1.2 × 104)

marmoset #4 (9.76 × 104)

marmoset #5 (7.95 × 105)

 

Plaque Assay (PFU/g)

PCR

Plaque Assay (PFU/g)

PCR

Plaque Assay (PFU/g)

PCR

Plaque Assay (PFU/g)

PCR

Plaque Assay (PFU/g)

PCR

Salivary Gland

-

-

-

-

1.4E + 04

-

-

-

-

-

Adrenal Gland

-

-

-

-

-

-

-

-

1.2E + 04

+

Pancreas

-

-

-

-

-

-

-

-

-

-

Lung

-

-

-

-

-

-

-

-

2.0E + 04

-

Spleen

-

-

-

-

-

+

-

+

-

-

Axillary LN

-

-

-

-

-

-

-

+

-

-

Kidney

-

-

-

-

-

+

1.3E + 04

+

9.5E + 03

-

Brain

-

-

-

+

1.6E + 07

+

4.2E + 04

+

-

+

Heart

-

-

-

-

-

-

3.6E + 03

-

-

+

Liver

-

-

-

-

-

+

1.9E + 04

-

-

+

Inguinal LN

-

-

-

-

4.2E + 04

-

-

-

-

-

Mandibular LN

-

-

-

-

4.2E + 04

+

-

-

7.4E + 03

-

Tracheobronchial LN

NT

NT

-

-

-

+

5.0E + 04

-

-

+

Mesentric LN

-

-

-

-

-

-

7.3E + 04

-

-

+

Popliteal LN

-

-

-

-

-

+

-

-

-

-

  1. NT no tissue
  2. + = positive assay result; − = negative assay result
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Virology Journal

ISSN: 1743-422X