Fig. 7

Immunohistochemistry analysis for marmosets exposed to aerosolized EEEV. The image in Panel a from the pons reveals widespread positive antigen staining of neurons in the brain, indicating the presence of EEEV viral RNA(immunohistochemistry; magnification, 40×). Panel b, immunohistochemical staining for the presence of antigen in the retina of marmoset #4. Depicted from the top of the image toward the bottom are: inner limiting membrane, optic fiber layer, ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, and external limiting membrane. The photo receptor layer appears predominantly negative (bottom of image) and the pigment epithelium is barely present in the image. Both images are from marmoset #4 exposed to 9.76 × 104 PFU of EEEV