Fig. 6

DENV 2 infection of HEK293T/17 cells with down-regulated fatty acid synthase (FASN). HEK293T/17 cells were treated with siRNAs targeted to FASN (FASN1 and FASN4) or a control siRNA (GFP) or not treated (DENV) and subsequently infected with DENV 2. At 24 h.p.i both percentage infection and virus titer were determined. Experiments were undertaken independently in triplicate, with duplicate plaque assay. (a to d) representative flow cytometry histograms with gating. M1: uninfected cell population, M2: infected cell population. Black line is mock infected (the same for all histograms), a red: DENV infected, b siGFP transfected and DENV infected, c siFASN1 transfected and DENV infected, d siFASN4 transfected and infected. e Bar chart showing M2 populations from three independent replicates and (f) bar chart showing results of plaque assay. Bars show mean +/−SD (*; p value <0.05)