Fig. 1From: Involvement of fatty acid synthase in dengue virus infectionObservation and quantification of neutral lipids by Oil red O staining. DENV 2 infected and mock infected (a) HEK293T/17 cells and (b) HepG2 cells cultured on glass microscope cover slips for 24 h were stained with Oil red O before observation under bright field of a Carl Zeiss LSM800 microscope. Oil droplets show as black circles. The smaller droplets in HEK293T/17 cells are arrowed. The staining was repeated on cells grown in six well plates for 24 and 36 h and Oil red O was subsequently eluted and quantified by spectrophotometry. Experiment was undertaken independently in triplicate. Bars show mean +/−SD (*; p value <0.05)Back to article page