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Table 5 q-PCR results for stability of the references subjected to freeze-thraw

From: Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

Reference

Ct value

First F-T

Second F-T

Third F-T

HB29 strain

25.49

26.23

27.31

26.29

25.80

26.58

26.10

26.28

25.80

Average

25.96

26.10

26.56

HB29 strain at 1:100

33.27

31.85

34.43

34.19

32.62

30.90

30.72

32.75

33.48

31.24

32.19

33.00

Average

32.36

32.35

32.95

  1. F-T freeze-thaw