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Table 5 q-PCR results for stability of the references subjected to freeze-thraw

From: Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

Reference Ct value
First F-T Second F-T Third F-T
HB29 strain 25.49 26.23 27.31
26.29 25.80 26.58
26.10 26.28 25.80
Average 25.96 26.10 26.56
HB29 strain at 1:100 33.27 31.85 34.43
34.19 32.62 30.90
30.72 32.75 33.48
31.24 32.19 33.00
Average 32.36 32.35 32.95
  1. F-T freeze-thaw