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Table 4 q-PCR results for stability of reference in different storage conditions

From: Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

References Ct value Normal value
37 °C for 8 days Room temperature for 14 days −70 °C for 8 months
Positive P1 (AH12) 18.02 22.81 22.84 18.60
P2 (HN1) 18.49 24.02 23.53 20.15
P3 (JS3) 24.83 25.07 25.26 20.84
P4 (LN3) 18.80 24.52 23.96 20.04
P5 (SD4) 22.41 23.88 24.05 19.27
P6 (HB29) 17.92 22.35 22.99 18.87
Sensitivity S1 (105U) 20.20 26.69 25.51 21.42
S2 (104U) 23.99 30.03 27.46 26.05
S3 (103U) 27.40 32.23 30.78 28.28
S4 (102U) 35.29 35.54 31.08 32.20
S5 (10U) 37.23 32.68
Accuracy R1 27.50 29.87 27.77 27.47
R2 24.56 29.78 28.08 27.08
R3 27.50 29.21 27.89 26.22
R4 24.30 30.61 27.89 28.15
R5 24.27 30.04 27.63 27.12
R6 23.99 28.70 27.85 25.56
R7 25.09 30.03 27.81 25.67
R8 25.11 31.76 28.01 24.84
R9 25.01 31.39 27.60 28.03
R10 25.06 31.16 27.37 25.09
SD 1.26 0.97 0.21 1.21
Average 25.24 30.26 27.79 26.52
CV 5.00% 3.20% 0.75% 4.55%