Table 1 Primers for complete genome amplification of ToMMV
Amplified fragments | sequence(5’-3’) | direction | site | length(bp) | Tm(°C) |
|---|---|---|---|---|---|
ToMMV1 | TTTAAGTATTTATTATTACAACAATTA | + | 1 ~ 1465 | 1465 | 54.2 |
GGACAGAAAGCTTTGTATGTAGG | - | ||||
ToMMV2 | TTTAAAGTCACCGCTAGGTCTGAGT | + | 1377 ~ 2816 | 1440 | 60.5 |
CCAGTGTGCAGCATCAATCC | - | ||||
ToMMV3 | GCTAAGGTTGTACTAGTAGACGG | + | 2551 ~ 3433 | 883 | 60.5 |
GTAATTGCTATTGAGTACCTGC | - | ||||
ToMMV4 | TTTAAACAGTCCCCATGTGCTTGTC | + | 3313 ~ 4481 | 1209 | 60.6 |
CCATCGGTAACATCGAGGCT | - | ||||
ToMMV5 | TTTAACTGGAGAAGACTGGGTCTAG | + | 4290 ~ 5565 | 1193 | 60.3 |
TTCGGTAAGTTCAATGGGACCT | - | ||||
ToMMV6 | TTTAAGGAACATATGGCAAGTCCTAGT | + | 5311 ~ 6398 | 1089 | 60.5 |
TGGGCCCCTACCGGGG | - | ||||
5RAGSPR1 | CAAGCGAGTGAACTGCGTTCTGAGTG | - | 1 ~ 325 | 325 | 69.5 |
5RAGSPR2 | GCCCGGGTAGCAATAAGTGTCTGTTC | - | 1 ~ 269 | 269 | 69.5 |
3RAGSPF1 | GTTAATGAATTGGTAAGAGGAACAGG TTTC | + | 6106 ~ 6398 | 293 | 66.7 |