Fig. 5
From: Interactions of human microglia cells with Japanese encephalitis virus
JEV detection and replication in human microglia. Human microglia were treated with Mock antigen and Nakayama isolate (used at an MOI of 10 TCID50/cell otherwise indicated) at 37 °C for various time periods. Virus detection and viral replication were evaluated. a Representative histogram plots of flow cytometry analysis showing intracellular JEV particles in human microglia (as gated in Fig. 1) after 48 h of exposure. Frequencies of JEV+-human microglia are indicated. b Transmission electron micrographs of Nakayama-treated human M-MG (right panel, magnification of 9700x) containing JEV particles (left panel, magnification of 66000x) after 24 h of exposure. Bars indicate the scale. Images are from a representative experiment of 2 independent experiments. c Curve lines of fold change in viral RNA compared to the time point 0 h using real-time RT-PCR. The marker represents the mean value and the error bar the standard deviation. Values are of a representative experiment with each condition performed in triplicate reaction