Fig. 4
From: Interactions of human microglia cells with Japanese encephalitis virus
Impact of JEV on MHC expression in human microglia. Human microglia were treated with Alum, JEV vaccine (used at a concentration of 1.2 pg/cell), Mock antigen, Nakayama and TC362 isolates (used at an MOI of 10 TCID50/cell) at 37 °C for 24 h. Expression of indicated MHC molecules on human microglia (gated as in Fig. 1) was investigated using flow cytometry. a, b Representative histogram plots showing the effect of JEV on MHC (a) class I and (b) class II. c Representative MHC class II versus CX3CR1 density plots of human M-MG (upper panel) and BdMG (lower panel) treated with JEV vaccine. d Representative MHC class II versus CX3CR1 density plots of human M-MG treated with live JEV. e Box plots showing the ratio of MHCIIhigh versus MHCIIlow on CX3CR1+-human M-MG subset. The black line represents the mean value and the error bars the standard deviation. Asterisks show significant differences between Alum and JEV vaccine or between Mock and the indicated live JEV isolate, calculated with the student t-test (* : p < 0.05; ** : p < 0.01; *** : p < 0.001)