Fig. 3
From: Interactions of human microglia cells with Japanese encephalitis virus
Impact of JEV on CX3CR1 expression in human microglia. Human microglia were treated with Alum, JEV vaccine (used at a concentration of 1.2 pg/cell otherwise indicated), Mock antigen, Nakayama and TC362 isolates (used at an MOI of 10 TCID50/cell otherwise indicated) at 37 °C for 24 h otherwise indicated. Expression of indicated chemokine receptor on human microglia (gated as in Fig. 1) was investigated using flow cytometry. a Upper panels are box plots of frequencies of the indicated chemokine receptor-expressing human microglia. The black line represents the mean value and the error bars the standard deviation. Lower panels are histogram bars of fold change in expression for the indicated chemokine receptor in human microglia. The bar represents the mean value; the error bars the standard. Values are of 3 independent experiments with each condition performed in triplicate cultures. b Representative histogram plots showing the effect of two different doses of JEV on CX3CR1 expression on human microglia. Low and high doses are respectively a concentration of 0.6 and 1.2 pg/cell in the context of JEV vaccine or an MOI of 1 and 10 TCID50/cell in the context of live JEV. c Curve lines of fold change in expression for CX3CR1 in human microglia upon treatment with JEV for various time periods. The marker represents the mean value and the error bar the standard deviation. Values are of a representative experiment with each condition performed in triplicate cultures. d Left panel displays representative SSC versus CX3CR1 density plots of human M-MG treated with a concentration 1.2 pg/cell in the context of JEV vaccine or an MOI of 10 TCID50/cell in the context of live JEV. Right panel displays representative SSC versus CX3CR1 density plots of human BdMG treated with a concentration 1.2 pg/cell in the context of JEV vaccine or an MOI of 0.1 TCID50/cell in the context of live JEV. The black gate frames the CX3CR1+ human microglia population and frequencies are indicated. Asterisks show significant differences between Alum and JEV vaccine or between Mock and the indicated live JEV isolate, calculated with the student t-test (* : p < 0.05; ** : p < 0.01; *** : p < 0.001)