Fig. 1
From: Interactions of human microglia cells with Japanese encephalitis virus
Morphology and cell death of JEV-treated human microglia. Human M-MG were treated with (a, b and e) Alum, JEV vaccine (used at a concentration of 1.2 pg/cell), (c, d and e) Mock antigen, Nakayama and TC362 isolates (used at a multiplicity of infection (MOI) of 10 TCID50/cell) at 37 °C for 24 h. Cell morphology and cell death were investigated. a, c Bright field micrographs (magnification of 20x) from a representative experiment of 3 independent experiments. b, d Flow cytometry analysis showing representative pseudo-colour plots of SSC versus FSC profile of human microglia observed in (a and c). Black gate delineates microglia cells excluding other cell types and cell debris. e Histogram bars presenting the levels of Annexin-V+-human microglia. Data are of 2 independent experiments with each condition performed in triplicate cultures. The bars represent the mean value; the error bars the standard deviation. Asterisks show significant differences between Alum and JEV vaccine or between Mock and the indicated live JEV isolate, calculated with the student t-test (* : p < 0.05; ** : p < 0.01; *** : p < 0.001)