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Table 1 Primers for genes of interest

From: Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome

UL83 forward 5′-TGTATCGCCG GAATTCTCAACCTCGGTGCTTTTTG-3′
reverse 5′-CTGCAGACGC GTCGACATGGAGTCGCGCGGTCGC-3′
AIM2 forward 5′-GTACGGTGGG GAATTCGGAGGCTGATCCCAAAGTTGT-3′
reverse 5′-ACGCGTCGAC GGATCCTGCTGCTTAGACCAGTTGGC-3′
ASC forward 5′-CTCAAGCTTC GAATTCATGGGGCGCGCGCGCGAC-3′
reverse 5′-CCGCGGTACC GTCGACTCAGCTCCGCTCCAGGTC-3′
caspase-1 forward 5′-CTCAAGCTTC GAATTCATGGCCGACAAGGTCCTG-3′
reverse 5′-CCGCGGTACC GTCGACTTAATGTCCTGGGAAGAGG-3′
IL-1β forward 5′-CTCAAGCTTC GAATTCATGGCAGAAGTACCTGAGC-3′
reverse 5′-CCGCGGTACC GTCGACTTAGGAAGACACAAATTGCAT-3′
  1. Italics: 15 bases homologous to one end of vector. Bold italics: restriction enzyme sites