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Fig. 1 | Virology Journal

Fig. 1

From: Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome

Fig. 1

Construction and expression of recombinant UL83 and AIM2 proteins. a UL83 ORF (1686 bp) was cloned into the MCS of the pM vector for the expression of a fusion of a bait protein (pUL83 herein) with Gal4 DNA BD (147 aa). b AIM2 ORF (1024 bp) was inserted into pVP16 vector to express recombinant AIM2 (the prey protein) fused to VP16 AD (45 aa). c Plasmids digested by EcoRI and SalI or EcoRI and BamHI and PCR products were analyzed by agarose gel electrophoresis. The sizes of the plasmid digestion and PCR products were as anticipated. d Verified recombinants were used to transfect HEK293T cells for 72 h. The expression of target proteins was assessed by SDS-PAGE with specific antibodies against pUL83 and AIM2. The expected recombinant protein sizes were 81 kDa (BD-pUL83) and 44 kDa (AD-AIM2)

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