Fig. 5
From: Host Cell Copper Transporters CTR1 and ATP7A are important for Influenza A virus replication
Localization of influenza nucleoprotein and copper transporter ATP7A in infected cells treated with exogenous copper of chelator. (a) A549 cells were untreated (Mock) or treated 24 hours with 50 μM CuCl2 or 10 μM TTM before infection with influenza A/WSN/33 (H1N1) virus (MOI = 1). Cells were fixed 12 h.p.i. for immunofluorescence microscopy to analyzing subcellular localization of NP. DAPI, blue; NP, green. Scale bar, 10 μm. (b) Immunofluorescence microscopy analyzing subcellular localization of ATP7A after CuCl2 or TTM at 12 h.p.i. DAPI, blue; TGN46, magenta; NP, red; ATP7A, green. Scale bar, 10 μm