Table 1 The primers for each immune factors and house-keeping gene
Gene | Primer sequence | Product of amplication (bp) |
|---|---|---|
FoxP3-F | TTTGCTGATTGTTGCTTTGC | |
FoxP3-R | GTTCAACTGATGCTGCCTGA | 158 |
CCL22-F | GGTATTTGAACCTGTGGAATTGGAG | |
CCL22-R | CAGGCCCTGGATGACACTGA | 163 |
CCR4-F | CCCTTAGGGATCATGCTGTT | |
CCR4-R | TCAAAGGTGCAGTCCTGAAG | 197 |
OX40-F | CAAGCCTGGAGTTGACTGTG | |
OX40-R | GATTGCGTCCGAGCTATTG | 139 |
Smad3-F | AGACCTCATGCCCAGCTCTC | |
Smad3-R | GGGGAGGGAGACAGACAAAAC | 165 |
OX40L-F | TCCCAGCCTCCAAACATAGT | |
OX40L-R | GTGTTGCTTTGCCTGTCTGT | 359 |
GAPDH-F | GCACCGTCAAGGCTGAGAAC | |
GAPDH-R | TGGTGAAGACGCCAGTGGA | 138 |