Skip to main content

Table 3 Primers and probes used for the microarray

From: Development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to Tomato spotted wilt virus

Primer/Probe

Length (bp)

Sequence (5’ → 3’)

Specificity

Primer name

   

 Pr-dTS-f

20

Biotin ATGTMTAAGGYHAAGHTYAC

TSWV serogroup

 Pr-dTS-r

26

Biotin GAAGCWATVAGAGGNADRCTWCCTCC

Probe name

   

 Pb-AN

40

T20 ATGGACTTCCTTTGAATGAT

ANSV

 Pb-CS

40

T20 ACGGGCTTAGCTTGAATGAT

CSNV

 Pb-GR

40

T20 ACGGGCTGCCTCTGGCAGAT

GRSV

 Pb-IN

40

T20 ATGGTCTTGCAACCACAGAT

INSV

 Pb-TS

40

T20 ATGGATTACCTCTCGATGAT

TSWV