Table 2 The primer pairs used for cloning of N genes of tospoviruses
Virus | Primer name | Sequence (5’ → 3’) |
|---|---|---|
ANSV | ANSV-Sph-Nde-N | GGCATGCGGCATATGTCTAAGGCTAAGTTGACAAGGGAA |
ANSV-Kpn-Xho-Nc | GCTCGAGTTAGGTACCAGCAACACCTGAAATTTTGGATTC | |
CSNV | CSN152Nco | GCCATGGCTAAAGTTAAGCTTACAAAGG |
CSN932cXho | GCTCGAGAACAAGATCTTTAGGAATAAG | |
GRSV | GR-Nc-N-2122 | GCCATGGCTAAGGTCAAGCTCACA |
GR-Xh-N-2895c | GCTCGAGTGCAACAACAGCAATCTT | |
INSV | IN2843cS | GGGCATGCATGAACAAAGCAAAGATTACCAAG |
IN2058K | AAGGTACCAATAGAATCATTTTTCCCAAAATC | |
TCSV | TC-Nc-N-f | GCCATGGCTAAGGTCAAGCTCACC |
TC-Xh-N-c | GCTCGAGTGCAACACCTGAAATTTT | |
TSWV | TN1990Xho | GCTCGAGAGCAAGTTCTGCGAGTTTTGC |
TN2763Ncoc | GCCATGGCTAAGGTTAAGCTCACTAAG |