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Fig. 3 | Virology Journal

Fig. 3

From: Development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to Tomato spotted wilt virus

Fig. 3

Sensitivity assay of the microarray. The PCR product amplified from pTOPO-TSWV-N using the primer pair Pr-dTS-f/Pr-dTS-r was diluted as shown for the test. a The diluted amplicons were analysed by agarose gel electrophoresis with ethidium bromide staining. b The microarray result. The concentrations of amplicon are indicated as 1: 250 ng; 2: 125 ng; 3: 62.5 ng; 4: 32 ng; 5: 16 ng; 6: 8 ng; 7: 4 ng; 8: 2 ng; 9: 1 ng; 10: 0.5 ng; 11: 0.2 ng; and 12: 0.1 ng

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