Fig. 3From: Development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to Tomato spotted wilt virus Sensitivity assay of the microarray. The PCR product amplified from pTOPO-TSWV-N using the primer pair Pr-dTS-f/Pr-dTS-r was diluted as shown for the test. a The diluted amplicons were analysed by agarose gel electrophoresis with ethidium bromide staining. b The microarray result. The concentrations of amplicon are indicated as 1: 250Â ng; 2: 125Â ng; 3: 62.5Â ng; 4: 32Â ng; 5: 16Â ng; 6: 8Â ng; 7: 4Â ng; 8: 2Â ng; 9: 1Â ng; 10: 0.5Â ng; 11: 0.2Â ng; and 12: 0.1Â ngBack to article page