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Fig. 1 | Virology Journal

Fig. 1

From: Development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to Tomato spotted wilt virus

Fig. 1

Design of degenerate primers for detection of the TSWV-serogroup tospoviruses. a Multiple alignments of the N gene sequences of ANSV, CSNV, GRSV, INSV, MeSMV, PNSV, TCSV, TSWV and ZLCV. The accession codes of individual sequences and virus abbreviations are listed in Table 1. The sequences of forward primer Pr-dTS-f and reverse primer Pr-dTS-r are shown in bold, and their corresponding positions of N gene sequences of individual tospoviruses are presented. The directions of primers (5’ to 3’) are indicated by arrows. b The primer pair Pr-dTS-f/Pr-dTS-r was used in a polymerase chain reaction for amplification of N gene fragments from the plasmids carrying the N gene sequences of ANSV, CSNV, GRSV, INSV, TCSV or TSWV. “-” represents a blank control

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Virology Journal

ISSN: 1743-422X