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Table 2 Validation of the OPV ABICAP assay in an external quality assessment for diagnostics of BT samples

From: Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

Virus

qPCR [CT]

Genome equivalents tested [GE/mL]

ABICAPa [A525nm]

Result

Monkeypox

27.0

9.2 × 105

1.570 ± 0.001

++

Vaccinia

22.5

2.4 × 107

2.032 ± 0.185

++

Vaccinia

30.7

6.2 × 104

0.437 ± 0.023

+

Vaccinia

38.0

2.3 × 103

0.119 ± 0.016

-

Ebola

23.5

2.7 × 106

0.169 ± 0.009

-

Marburg

23.3

8.2 × 106

0.152 ± 0.050

-

Yellow fever

n.d.b

-

0.194 ± 0.004

-

Cell culture medium (DMEM)

-

-

0.181 ± 0.005

-

  1. aMean ± standard deviation of n = 2
  2. bNot determined