A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox masquinongy), rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta), and lake trout (Salvelinus namaycush)

Table 2 Results of vaccination trials in three salmonid species

Treatment	Fish	Cumulative Mortality	RPS	χ²	P-value
RBT-1
pcDNA	29	62.1%
pVHSivb-G	26	26.9%	56.7%	6.83	0.009
pVHSivb-G (2 doses)	13	15.4%	75.2%	7.84	0.005
RBT-2
pcDNA	35	8.6%
pVHSivb-G	17	0.0%	100.0%	1.55	0.214
pVHSivb-G (2 doses)	31	0.0%	100.0%	2.78	0.095
BNT-1
pcDNA	26	23.1%
pVHSivb-G	38	5.3%	77.2%	4.48	0.034
pVHSivb-G (2 doses)	18	16.7%	27.8%	0.27	0.604
BNT-2
pcDNA	28	10.7%
pVHSivb-G	25	4.0%	62.7%	0.85	0.356
pVHSivb-G (2 doses)	25	4.0%	62.7%	0.85	0.356
LAT-1
pcDNA	26	30.7%
pVHSivb-G	34	0.0%	100.0%	12.07	0.0005
pVHSivb-G (2 doses)	27	0.0%	100.0%	9.79	0.0018
LAT-2
pcDNA	26	34.6%
pVHSivb-G	34	0.0%	100.0%	13.85	0.0002
pVHSivb-G (2 doses)	27	0.0%	100.0%	11.26	0.0008

Rainbow trout (RBT-1 and RBT-2), brown trout (BNT-1 and BNT-2) and lake trout (LAT-1 and LAT-2) vaccine trials. Table includes the number of fish in each treatment, the cumulative mortality and the relative percent survival (RPS) and the associated P-value calculated using a two-tailed χ²-test

ISSN: 1743-422X