A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge ( Esox masquinongy ), rainbow trout ( Oncorhynchus mykiss ), brown trout ( Salmo trutta ), and lake trout ( Salvelinus namaycush )

Table 2 Results of vaccination trials in three salmonid species

Treatment	Fish	Cumulative Mortality	RPS	χ²	P-value
RBT-1
pcDNA	29	62.1%
pVHSivb-G	26	26.9%	56.7%	6.83	0.009
pVHSivb-G (2 doses)	13	15.4%	75.2%	7.84	0.005
RBT-2
pcDNA	35	8.6%
pVHSivb-G	17	0.0%	100.0%	1.55	0.214
pVHSivb-G (2 doses)	31	0.0%	100.0%	2.78	0.095
BNT-1
pcDNA	26	23.1%
pVHSivb-G	38	5.3%	77.2%	4.48	0.034
pVHSivb-G (2 doses)	18	16.7%	27.8%	0.27	0.604
BNT-2
pcDNA	28	10.7%
pVHSivb-G	25	4.0%	62.7%	0.85	0.356
pVHSivb-G (2 doses)	25	4.0%	62.7%	0.85	0.356
LAT-1
pcDNA	26	30.7%
pVHSivb-G	34	0.0%	100.0%	12.07	0.0005
pVHSivb-G (2 doses)	27	0.0%	100.0%	9.79	0.0018
LAT-2
pcDNA	26	34.6%
pVHSivb-G	34	0.0%	100.0%	13.85	0.0002
pVHSivb-G (2 doses)	27	0.0%	100.0%	11.26	0.0008

Rainbow trout (RBT-1 and RBT-2), brown trout (BNT-1 and BNT-2) and lake trout (LAT-1 and LAT-2) vaccine trials. Table includes the number of fish in each treatment, the cumulative mortality and the relative percent survival (RPS) and the associated P-value calculated using a two-tailed χ²-test