A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge ( Esox masquinongy ), rainbow trout ( Oncorhynchus mykiss ), brown trout ( Salmo trutta ), and lake trout ( Salvelinus namaycush )

Table 1 Summary of the three trials conducted using muskellunge (MUS-1, 2, 3)

	Fish	Cumulative mortality	Mean Cumulative Mortality	Mean day to death ± SE	HR (95%CI)	RPS	P-value
MUS-1
pVHSivb-G	10	0%	5%	NA	0.016 (0.002–0.124)	95%	0.003
pVHSivb-G	10	10%	5%	NA	0.016 (0.002–0.124)	95%	0.003
pcDNA	10	100%	100%	11.7 ± 1.3		NA	NA
pcDNA	10	100%	100%	11.7 ± 1.3		NA	NA
MUS-2
pVHSivb-G	10	30%	15%	10.6 ± 0.4	0.063 (0.018–0.223)	85%	0.030
pVHSivb-G	10	0%	15%	10.6 ± 0.4	0.063 (0.018–0.223)	85%	0.030
pcDNA	10	100%	100%	9.9 ± 1.0		NA	NA
pcDNA	10	100%	100%	9.9 ± 1.0		NA	NA
MUS-3
pVHSivb-G	10	0%	0%	NA	NA	100%	<0.0001
pVHSivb-G	10	0%	0%	NA	NA	100%	<0.0001
pcDNA	10	100%	100%	12.8 ± 1.8		NA	NA

Total number of fish challenged in each trial, mean cumulative % mortality, and mean days to death of each treatment. The table also includes mean day to death calculated using PROC LIFETEST in SAS [22]. A Cox proportional hazard model was also fitted using SAS to examine the hazard ratio (HR) between glycoprotein (G) gene and mock-vaccinated counterparts. The relative percent survival (RPS) was calculated between treatments as previously described [23]. The P-values are from t-tests testing survival differences between the treatments that were conducted using PROC TTEST in SAS