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Table 1 Summary of the three trials conducted using muskellunge (MUS-1, 2, 3)

From: A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox masquinongy), rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta), and lake trout (Salvelinus namaycush)

  Fish Cumulative mortality Mean Cumulative Mortality Mean day to death ± SE HR (95%CI) RPS P-value
MUS-1
 pVHSivb-G 10 0% 5% NA 0.016 (0.002–0.124) 95% 0.003
 pVHSivb-G 10 10%
 pcDNA 10 100% 100% 11.7 ± 1.3   NA NA
 pcDNA 10 100%
MUS-2
 pVHSivb-G 10 30% 15% 10.6 ± 0.4 0.063 (0.018–0.223) 85% 0.030
 pVHSivb-G 10 0%
 pcDNA 10 100% 100% 9.9 ± 1.0   NA NA
 pcDNA 10 100%
MUS-3
 pVHSivb-G 10 0% 0% NA NA 100% <0.0001
 pVHSivb-G 10 0%
 pcDNA 10 100% 100% 12.8 ± 1.8   NA NA
  1. Total number of fish challenged in each trial, mean cumulative % mortality, and mean days to death of each treatment. The table also includes mean day to death calculated using PROC LIFETEST in SAS [22]. A Cox proportional hazard model was also fitted using SAS to examine the hazard ratio (HR) between glycoprotein (G) gene and mock-vaccinated counterparts. The relative percent survival (RPS) was calculated between treatments as previously described [23]. The P-values are from t-tests testing survival differences between the treatments that were conducted using PROC TTEST in SAS