A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox masquinongy), rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta), and lake trout (Salvelinus namaycush)

Table 1 Summary of the three trials conducted using muskellunge (MUS-1, 2, 3)

	Fish	Cumulative mortality	Mean Cumulative Mortality	Mean day to death ± SE	HR (95%CI)	RPS	P-value
MUS-1
pVHSivb-G	10	0%	5%	NA	0.016 (0.002–0.124)	95%	0.003
pVHSivb-G	10	10%	5%	NA	0.016 (0.002–0.124)	95%	0.003
pcDNA	10	100%	100%	11.7 ± 1.3		NA	NA
pcDNA	10	100%	100%	11.7 ± 1.3		NA	NA
MUS-2
pVHSivb-G	10	30%	15%	10.6 ± 0.4	0.063 (0.018–0.223)	85%	0.030
pVHSivb-G	10	0%	15%	10.6 ± 0.4	0.063 (0.018–0.223)	85%	0.030
pcDNA	10	100%	100%	9.9 ± 1.0		NA	NA
pcDNA	10	100%	100%	9.9 ± 1.0		NA	NA
MUS-3
pVHSivb-G	10	0%	0%	NA	NA	100%	<0.0001
pVHSivb-G	10	0%	0%	NA	NA	100%	<0.0001
pcDNA	10	100%	100%	12.8 ± 1.8		NA	NA

Total number of fish challenged in each trial, mean cumulative % mortality, and mean days to death of each treatment. The table also includes mean day to death calculated using PROC LIFETEST in SAS [22]. A Cox proportional hazard model was also fitted using SAS to examine the hazard ratio (HR) between glycoprotein (G) gene and mock-vaccinated counterparts. The relative percent survival (RPS) was calculated between treatments as previously described [23]. The P-values are from t-tests testing survival differences between the treatments that were conducted using PROC TTEST in SAS

ISSN: 1743-422X