
Fish

Cumulative mortality

Mean Cumulative Mortality

Mean day to death ± SE

HR (95%CI)

RPS

Pvalue


MUS1

pVHSivbG

10

0%

5%

NA

0.016 (0.002–0.124)

95%

0.003

pVHSivbG

10

10%

pcDNA

10

100%

100%

11.7 ± 1.3
 
NA

NA

pcDNA

10

100%

MUS2

pVHSivbG

10

30%

15%

10.6 ± 0.4

0.063 (0.018–0.223)

85%

0.030

pVHSivbG

10

0%

pcDNA

10

100%

100%

9.9 ± 1.0
 
NA

NA

pcDNA

10

100%

MUS3

pVHSivbG

10

0%

0%

NA

NA

100%

<0.0001

pVHSivbG

10

0%

pcDNA

10

100%

100%

12.8 ± 1.8
 
NA

NA

 Total number of fish challenged in each trial, mean cumulative % mortality, and mean days to death of each treatment. The table also includes mean day to death calculated using PROC LIFETEST in SAS [22]. A Cox proportional hazard model was also fitted using SAS to examine the hazard ratio (HR) between glycoprotein (G) gene and mockvaccinated counterparts. The relative percent survival (RPS) was calculated between treatments as previously described [23]. The Pvalues are from ttests testing survival differences between the treatments that were conducted using PROC TTEST in SAS