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Fig. 4 | Virology Journal

Fig. 4

From: Construction of a molecular clone of ovine enzootic nasal tumor virus

Fig. 4

Deletion of a proline residue from the polyproline tract in the matrix protein results in production of fully processed ENTV-1 virions. a ClustalW alignment of variable region 1 (VR1) of the matrix protein from a European isolate of ENTV-1 (ENTV-1EU, accession number NC 007015), all ten ENTV-1 isolates from North America (ENTV-1NA, see Table 1 [19] for accession numbers), ENTV-2 (accession number NC 004994), JSRV (accession number AF105220) and an endogenous JSRV (enJS56A1, accession number AF153615). Dots indicate identical amino acids and dashes indicate deletions. Nonconserved proline residues are highlighted in red. b Immunoblot analysis of capsid in cell lysates or concentrated virus particles derived from the JSRV molecular clone (pCMVJSRV21), the ENTV-1 molecular clone with the S199P mutation (pCMVENTV-1S199P), and the final version of the ENTV-1 molecular clone with the S199P and the delta Pro mutation (pCMVENTV-1S199PGagΔP) after transfection of HEK 293 T cells. M indicates the lane containing the molecular weight marker and lanes labelled as ECa contain bacterially expressed and purified ENTV-1 capsid protein. c and d Two representative examples of transmission electron microscope analysis of recombinant ENTV-1 particles. e Transmission electron microscope analysis of control grid coated with concentrated cell-free supernatant derived from mock transfected cells

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