Sample | Initial read count | Final read count (%) | Minimum mean QC (Phred) | Range of fragment length |
---|
BA3NI-I0 | 1,155,428 | 817,773 (70.8%) | 27 | 75–136 |
BA3NI-I6-PI | 1,052,378 | 793,149 (75.4%) | 27 | 75–136 |
BA3NI-I9 | 1,083,813 | 799,653 (73.8%) | 27 | 75–136 |
- Reads were preprocessed by trimming 15 nucleotides from the 5’ end, and then by trimming both ends using a sliding window of 3 nucleotides and an average Phred score of 25. This was followed by filtering reads such that 80% or more nucleotides had quality score over 20 and removing all reads with length less than 75