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Fig. 6 | Virology Journal

Fig. 6

From: Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infection

Fig. 6

Multiple rounds of selection with NDV decrease susceptibility of BA3NI to NDV cytolytic effect at 72 hpi. After nine rounds of selection, BA3NI-C1-I9 (a, b) and BA3NI-C2-I9 (c, d) show decreased susceptibility to NDV cytolytic effect compared to BA3NI-I0 (never infected cells) (e, f). Cell counting shows that BA3NI-C1-I9 and BA3NI-C2-I9 have roughly 36 and 45 times more cells than naïve infected cells at 72hpi (g). D-1, day of cell plating; D0, time of infection; D3, 72hpi (day 3 post infection). For each experiment, n = 3. Counting values are expressed as mean ± SD. Letters indicate significantly different values at the same time point (two-way ANOVA for repeated measurements, Tukey’s test for multiple comparisons, p < 0.05)

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Virology Journal

ISSN: 1743-422X