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Fig. 4 | Virology Journal

Fig. 4

From: Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infection

Fig. 4

Iterative process for ciPSCs selection. At D-1 cells were plated at 1.6X105 cells/well, and infected at D0 (time of infection, TOI) with NDV strain LS-RFP at MOI = 50. At D3 (3 days post infection [dpi]), cells were washed, expanded and propagated for another round of infection. Every 3 rounds of infection, a representative sample of the selected cells was infected with the same methodology to assess putative progression of tolerance

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