Fig. 4From: Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infectionIterative process for ciPSCs selection. At D-1 cells were plated at 1.6X105 cells/well, and infected at D0 (time of infection, TOI) with NDV strain LS-RFP at MOI = 50. At D3 (3 days post infection [dpi]), cells were washed, expanded and propagated for another round of infection. Every 3 rounds of infection, a representative sample of the selected cells was infected with the same methodology to assess putative progression of toleranceBack to article page