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Fig. 3 | Virology Journal

Fig. 3

From: Derivation of chicken induced pluripotent stem cells tolerant to Newcastle disease virus-induced lysis through multiple rounds of infection

Fig. 3

ciPSCs demonstrate rapid recovery after infection with NDV. Photomicrographs showing BA3NI (panels a, b) and DF-1 (panels c, d) cells at day 6 post-infection (144 hpi) with rLS-RFP at MOI = 50. BA3NI appear as large confluent colonies, as opposed to DF-1, which do not increase in number after infection. Panels ad, magnification 20X; insets, magnification 10X; panels a, c bright field, b, d red fluorescence. A representative counting of infected DF-1 compared to infected BA3NI is shown in the graph (panel e). D-1, day of cell plating; D0, time of infection; D3, 72 hpi (day 3 post-infection); D6, 144 hpi (day 6 post-infection). For each experiment, n = 3. Counting values are expressed as mean ± SD. *indicates significant differences between average number of cells at each time point (two-way ANOVA for repeated measurements, Sidak’s test for multiple comparisons, p < 0.05)

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