Fig. 5
Antigen-specific immunoglobulin responses to linear SIVMAC239 Gag, Nef and Tat peptides. Amino acid sequences of SIVMAC239 Gag (a) and Nef (e) proteins, indicating regions containing potential linear epitopes responsible for peptide-specific IgG, IgM and/or IgA responses were shown. Bracketed regions identify the amino acid sequences used to construct 15mer 10 peptide pools (PP1-13 for Gag and PP1-7 for Nef proteins). Colors refer to the percentage of animals exhibiting peptide-specific immunoglobulins for that region during infection with SIVMAC251 as shown in the key. The Gag amino acid sequence (a) was divided into 6 proteins (p15, p27, p2, p8, p1 and p6) with two important regions, p8 (nucleocapsid region) and p6, shown in shaded area. The percentage of macaques with SIV-Gag peptide-specific IgG (b), IgM (c) and IgA (d) responses, SIV-Nef peptide-specific IgG (f) and IgM (g) and SIV-Tat peptide-specific IgG (h) and IgM (i) responses were shown for SIV infected macaques inoculated either intravaginally (n = 6) or intrarectally (n = 7). Responses to individual peptide pools (PP) were shown for each time point (days post infection; dpi). Colors refer to the percentage of animals exhibiting peptide-specific immunoglobulin responses for that region during infection with SIVMAC251 as shown in the key. Note that Nef- and Tat-specific IgA responses were not detected