Fig. 1

A2 from macrophage lysates is captured on HIV-1 gp120-coated SiMPull slides. Lysis buffer (a) or macrophage cell lysates (b) were flowed onto SiMPull slides coated with increasing amounts of biotinylated gp120, and the number of captured complexes (c) were detected following staining with a rabbit anti-A2 antibody and an anti-rabbit 568-conjugated secondary antibody using TIRF microscopy, where each white dot represents one protein-protein complex (scale bar = 5 μm). Controls included no gp120 and no lysate. Data are presented as the means ± SD of five fields of view of a representative example of an experiment performed three times. *p < 0.05 **p < 0.01 as determined by a one-way ANOVA followed by a Kruskal-Wallis multiple comparisons test against the no gp120 control group. d In a separate experiment, lysates were flowed onto SiMPull slides coated with an anti-A2 antibody, and captured complexes were detected with mouse anti-S100A10 or anti-SLPI primary antibodies and an anti-mouse 568-conjugated secondary antibody. ***p < 0.001 as determined by an unpaired two-tailed Student’s T-test against the no capture control group