Skip to main content

Table 1 List of primers used for developing multiplex RT-PCR

From: Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction

Primer

Primer sequence (5′ to 3′)

Positions

BrYA484F

5′- TACTTGGACTAGAGATGCTGAAAG-3′

BrYV-A 484–507 nt

BrYB88F

5′- CCTCCACCCAAAACAAGTAT-3′

BrYV-B 88–107 nt

BrYC257F

5′- CGAGTTTCCGTACTTGTTG-3′

BrYV-C 257–275 nt

BrY761R

5′- AGACCGAAGAGCTGAAAAGG-3′

BrYV-A 742–761 nt

TuY4959F

5′- AAGAGGCTTGCCCCTTCCTG-3′

TuYV 4959–4978 nt

TuY5163R

5′- AACCAAATCCGGTGTTGGAT-3′

TuYV 5144–5163 nt