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Table 1 List of primers used for developing multiplex RT-PCR

From: Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction

Primer Primer sequence (5′ to 3′) Positions
BrYA484F 5′- TACTTGGACTAGAGATGCTGAAAG-3′ BrYV-A 484–507 nt
BrYB88F 5′- CCTCCACCCAAAACAAGTAT-3′ BrYV-B 88–107 nt
BrYC257F 5′- CGAGTTTCCGTACTTGTTG-3′ BrYV-C 257–275 nt
BrY761R 5′- AGACCGAAGAGCTGAAAAGG-3′ BrYV-A 742–761 nt
TuY4959F 5′- AAGAGGCTTGCCCCTTCCTG-3′ TuYV 4959–4978 nt
TuY5163R 5′- AACCAAATCCGGTGTTGGAT-3′ TuYV 5144–5163 nt