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Fig. 3 | Virology Journal

Fig. 3

From: Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction

Fig. 3

Specificity of the multiplex RT-PCR for detection of BrYV-A, BrYV-B, BrYV-C, and TuYV. Lanes 1 show healthy Nicotiana benthamiana . Lane 2, 100 bp DNA marker. Lanes 3–5 show amplification of individual BrYV genotype products. Lane 6 shows pMD19-TuYV-P0 and pMD19-56#-1. Lane 7 shows BrYV-A, BrYV-B and BrYV-C co-infected N. benthamiana. Lane 8 shows the BrYV-A, BrYV-B and BrYV-C co-infected N. benthamiana plus pMD19-TuYV-P0 and pMD19-56#-1. Lanes 9–12 show the plasmids containing genome sequences of Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV), Cucurbit aphid-borne yellows virus (CABYV) and Beet western yellows virus Inner Mongolia isolate (BWYV-IM) infectious cDNA clones

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Virology Journal

ISSN: 1743-422X