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Fig. 2 | Virology Journal

Fig. 2

From: Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction

Fig. 2

Detection limit of multiplex RT-PCR using ten-fold serial dilutions of individually plasmids. (a, b, c, e), uniplex PCR; (d, f), multiplex PCR. Lane M, 100 bp DNA marker. Lanes 1–8, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg of plasmids of different BrYV genotypes. (e), pMD19-TuYV-P0 and pMD19-56#-1 were used for dectection of TuYV

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