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Fig. 1 | Virology Journal

Fig. 1

From: Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction

Fig. 1

Optimization of the annealing temperature for uniplex RT-PCR of (a) BrYV-A (278 bp); (b) BrYV-B (674 bp); (c) BrYV-C (505 bp); (d) multiplex RT-PCR assay and (e) TuYV (205 bp) . (a, b, c, e), uniplex RT-PCR; (d), multiplex RT-PCR. Lane M: 100 bp DNA Marker; Lanes 1–8, 60.0 °C, 60.6 °C, 61.4 °C, 62.4 °C, 63.8 °C, 64.9 °C, 65.5 °C, 66.0 °C

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Virology Journal

ISSN: 1743-422X