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Fig. 6 | Virology Journal

Fig. 6

From: Identification of molecular determinants of cell culture growth characteristics of Enterovirus 71

Fig. 6

Western blot analysis of VP1 formed by the various EV-A71 mutants. The amount of EV-A71 VP1 protein and β-actin loaded in each lane was 5 μg. The lanes are labelled as follows: lane 1, EV-A71 Mutant 158; lane 2, EV-A71 Mutant 475; lane 3, EV-A71 Mutant 486; lane 4, EV-A71 Mutant 487; lane 5, EV-A71 Mutant 5262; lane 6, EV-A71 Mutant Partial Deletant (PD) 5′-NTR; lane 7, Positive control (EV-A71 Virus); lane 8, Negative control (uninfected RD cells). The molecular weight of the EV-A71 VP1 protein is estimated to be 32.7 kDa with reference to molecular weight markers (from 10–250 kDa) (data not shown)

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