Fig. 2
From: Identification of molecular determinants of cell culture growth characteristics of Enterovirus 71
Cytopathic effects (CPE) caused by mutants a EV-A71 (A158T) b C475T c A486G d G487A e A5262G and f PD (Partial Deletant, 5′-NTR) in Rhabdomyosarcoma (RD) cells in comparison with g uninfected RD cells (negative control using Opti-MEM) and h EV-A71 wild type strain 41 infected RD cells (positive control). Transfection of infectious RNA into RD cells was performed with the use of Lipofectamine 2000 reagent using EV-A71 mutants at a MOI of 0.1. CPE was seen as round and shrunken cells that eventually dislodged from the surface. Magnification used (X 100)