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Fig. 4 | Virology Journal

Fig. 4

From: Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions

Fig. 4

Targeting of pUL34 to the nuclear envelope during infection is independent of Asna1/TRC40. To determine the importance of Asna1/TRC40 for localization of pUL34 to the nuclear envelope in the virus context, HeLa cells were transfected with Asna1/TRC40 specific and control (ctrl) siRNA for 48 h (a). HSV1(F) infection was then performed for 12 h at an MOI of 0.1 followed by IF analysis using rabbit anti-pUL34 antibodies (b) or goat anti-Lamin B antibodies (c) in combination with mouse anti-ICP8 antibodies and secondary reagents. Nuclei were visualized by DAPI staining

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