Fig. 3

Asna1/TRC40 is dispensable for virion entry and gene expression during HSV1 infection. a To determine the importance of Asna1/TRC40 for entry and early gene expression of HSV1, HeLa cells were transfected with Asna1/TRC40 specific or control (ctrl) siRNA for 48 h. Silencing was monitored as described in Fig. 2a (inset). Subsequently, cells were infected with HSV1(F) at an MOI of 0.5 for 4 h and analysed by IF using monoclonal anti-ICP0 antibody followed by secondary reagents. Nuclei were visualized by DAPI staining. A total of 700 cells of either cell type was analysed. b To determine whether Asna1/TRC40 is required for overall viral gene expression, cells treated with siRNA for 48 h were subsequently infected with HSV1 at an MOI of 5, harvested at the indicated times post infection and analysed by Western blotting using ICP0, ICP27, glycoprotein B (gB), pUL34 and VP5 (ICP5), and specific antibodies followed by secondary reagents. For control, β-actin specific antibodies were used