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Fig. 2 | Virology Journal

Fig. 2

From: Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions

Fig. 2

Targeting and membrane insertion of HSV1 pUL34, pUL56 and pUS9 occur independent of Asna1/TRC40. a To determine the effect of Asna1/TRC40 depletion on cell growth, HeLa cells were transfected with Asna1/TRC40 specific or control (ctrl) siRNA for 48 h. Gene knockdown was monitored by Western blotting using Asna1/TRC40 and Lamin B specific antibodies followed by secondary reagents. While efficient knock-down was achieved, cell viability was normal indicating that Asna1/TRC40 silencing did not affect cellular growth. b To determine whether the HSV1 TA proteins require the Asna1/TRC40 mediated pathway for localization to their target membrane, Asna1/TRC40 knockdown was performed in HeLa cells. Subsequently, cells were transfected with plasmids encoding myc-tagged TA proteins and 20 h later analysed by IF using monoclonal anti-myc and polyclonal anti-Calreticulin or anti-Giantin antibodies followed by secondary reagents. Nuclei were visualized by DAPI staining

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