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Fig. 6 | Virology Journal

Fig. 6

From: Activation of HIV-1 expression in latently infected CD4+ T cells by the small molecule PKC412

Fig. 6

Additive effect of PKC412 and VOR on the activation of latent HIV-1 expression. a ACH2 cells were treated with different concentrations of PKC412 and/or VOR. After 48 h, the activating effect of PKC412 and/or VOR on HIV expression was determined by measuring the HIV p24 level in the supernatants. b HIV transcript levels in ACH2 cells treated with PKC412 and/or VOR were measured with real-time PCR using primers corresponding to the HIV 5′LTR and gag (R-gag) and calculated as relative HIV-1 mRNA levels to non-treated ACH2 cells. c The schematic diagram of HIV infection in resting CD4+ T cells and the effects of PKC412 and/or VOR. d The infected resting CD4+ T cells from different donors were treated with PKC412 and/or VOR or untreated for 2 days. Then, the HIV Gag p24 levels from the supernatants were monitored by ELISA and calculated as relative P24 levels compared to the infected-untreated cells. The experiments were performed as triplicate repeats per assay for each donor. The mean values from each donor are shown with the standard error of the mean (SEM). The degree of significance for PKC412 or VOR treatment was relative to the infected-untreated cells. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001

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