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Fig. 3 | Virology Journal

Fig. 3

From: Activation of HIV-1 expression in latently infected CD4+ T cells by the small molecule PKC412

Fig. 3

The effect of PKC412 on HIV-1 re-activation is independent of PKC412-induced cell cycle G2 arrest. a ACH2 cells were treated with PKC412 (0.5 μM) for 24 h and the cell cycle profiles were measured by staining with propidium iodide (PI) and analyzed with flow cytometry. b After 24 or 48 h, the cells were fixed and visualized under a microscope (20× magnification). c ACH2 cells were treated with aphidicolin (2 μM) for 12 h and then PKC412 (0.5 μM) for another 24 h. The cell cycle profiles of the ACH2 cells were analyzed with flow cytometry. The plot shows cells in G0/G1 phase (Green), S phase (yellow) and G2/M phase (blue). d ACH2 cells were treated with aphidicolin (2 μM) and PKC412 (0.5 μM) alone or in combination. After 48 h, the HIV-1 p24 level in each well was measured by HIV-1 p24 ELISA. Error bars represent variations between duplicate samples and the data are representative of results obtained in three independent experiments. The results were significant (* p < 0.05 and ** p < 0.01) compared to the mock treated cells

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