Fig. 1

PKC412 stimulates HIV-1 expression in latently infected ACH2 cells. a A over 1,500 small molecules and kinase inhibitors were tested in HIV latently infected ACH2 cells in 96-well plates at a final concentration of 2 μM. After two days, the HIV-1 p24 level in each well was measured by ELISA. b PKC412 chemical structure. c ACH2 cells cultured in RPMI medium containing 1 % or 10 % FBS were treated with PKC412 at different concentrations for 48 h; then, HIV p24 production was measured in the cell culture supernatants. Error bars represent variations between duplicate samples and the data are representative of results obtained in three independent experiments. d Assessment of PKC412 cytotoxicity by the trypan blue dye exclusion assay. ACH2 cells in 1 % or 10 % FBS medium and human resting CD4+ T cells were treated with different PKC412 concentrations. After 48 h, the cells were assessed using the trypan blue dye exclusion assay and counted using a TC20 Automated Cell Counter. Error bars represent variation between duplicate samples and the data are representative of results obtained in three independent experiments