Table 1 Viral DNA content in THP-1 latency and reactivation models (two independent experiments) and standard deviations
From: Human cytomegalovirus reactivation from latency: validation of a “switch” model in vitro
Times of infection | MOI (PFU/cell) | THP-1 latency model | THP-1 reactivation model | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
DNA copies/ml | DNA copie/ml (log10) | SDa (log10) | DNA copies/ml | DNA copie/ml (log10) | SDa (log10) | ||||||
1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | ||||
30 h | 0.125 | 1.32 × 104 | 1.29 × 104 | 4.121 | 4.111 | 0.007 | 1.44 × 104 | 1.95 × 104 | 4.158 | 4.290 | 0.094 |
0.25 | 2.04 × 104 | 2.21 × 104 | 4.309 | 4.344 | 0.024 | 2.13 × 104 | 2.69 × 104 | 4.328 | 4.430 | 0.072 | |
0.5 | 6.51 × 104 | 6.80 × 104 | 4.813 | 4.832 | 0.013 | 5.80 × 104 | 6.09 × 104 | 4.763 | 4.785 | 0.015 | |
4 days | 0.125 | 8.96 × 103 | 8.12 × 103 | 3.952 | 3.909 | 0.030 | 2.44 × 104 | 2.30 × 104 | 4.387 | 4.362 | 0.018 |
0.25 | 2.81 × 104 | 2.91 × 104 | 4.449 | 4.464 | 0.010 | 5.56 × 104 | 5.80 × 104 | 4.745 | 4.763 | 0.013 | |
0.5 | 4.71 × 104 | 4.95 × 104 | 4.673 | 4.695 | 0.015 | 1.32 × 105 | 1.54 × 105 | 5.120 | 5.187 | 0.046 | |
6 days | 0.125 | 6.31 × 103 | 6.91 × 103 | 3.800 | 3.839 | 0.028 | 1.53 × 105 | 1.27 × 105 | 5.185 | 5.104 | 0.056 |
0.25 | 1.89 × 104 | 1.75 × 104 | 4.277 | 4.243 | 0.023 | 2.97 × 105 | 2.50 × 105 | 5.473 | 5.398 | 0.053 | |
0.5 | 2.75 × 104 | 2.61 × 104 | 4.440 | 4.417 | 0.016 | 7.30 × 105 | 6.50 × 105 | 5.863 | 5.813 | 0.035 | |
7 days | 0.125 | 4.80 × 103 | 4.21 × 103 | 3.681 | 3.624 | 0.040 | 1.32 × 106 | 1.70 × 106 | 6.120 | 6.230 | 0.077 |
0.25 | 1.50 × 104 | 1.30 × 104 | 4.176 | 4.113 | 0.044 | 5.58 × 106 | 5.42 × 106 | 6.747 | 6.734 | 0.009 | |
0.5 | 2.03 × 104 | 2.14 × 104 | 4.307 | 4.330 | 0.016 | 9.08 × 106 | 1.02 × 107 | 6.958 | 7.006 | 0.034 | |