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Fig. 2 | Virology Journal

Fig. 2

From: Human cytomegalovirus reactivation from latency: validation of a “switch” model in vitro

Fig. 2

Analysis of HCMV transcripts from THP-1 latency and reactivation models at 7 days p.i.. The expression patterns of the anti-sense CLT (cytomegalovirus latency associated transcript), IE1 (immediate-early), DNA pol (early), pp65 (early-late) and pp150 (late) viral transcripts were analysed in both models (panels “THP-1 latency model” and “THP-1 reactivation model”) after infection at three different MOI. The cellular GAPDH housekeeping gene amplification product was used as a reaction control. Lanes 1, 2 and 3: MOI 0.5, 0.25, 0.125, respectively; lanes 4, 5 and 6: DNA contamination controls (PCR performed for MOI 0.5, 0.25, 0.125, respectively, in the absence of reverse transcription). Molecular weights of the amplified transcript fragments are shown on the right side. The results are representative of two independent experiments

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