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Fig. 3 | Virology Journal

Fig. 3

From: The speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors

Fig. 3

Nonstructural protein P7-1 fused with Strep-tag of Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV) expressed in Sf9 cells. At 18 (I), 24 (II), 36 (III) and 48 (IV) h post-inoculation, the recombinant (a) RBSDV and (b) SRBSDV P7-1 proteins fused with strep-tag were detected by immunofluorescence with a monoclonal anti-Strep-tag antibody and anti-mouse IgG conjugated with FITC. The recombinant (c) RBSDV and (d) SRBSDV P7-1 proteins fused with strep-tag were stained with RBSDV P7-1-FITC and SRBSDV P7-1-FITC, respectively. Scale bars, 5 μm

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