Fig. 2

Immunofluorescent detection of Southern rice black-streaked dwarf virus (SRBSDV) P7-1 tubules in viruliferous white-backed planthoppers (WBPHs). The internal organs of viruliferous WBPHs at 1, 2, 4 and 6-day post-first access to diseased plants (padp) were immunolabeled with P7-1-FITC (green), P9-1-rhodamine (red) and the actin dye phalloidin-Alexa Fluor 633 carboxylic acid (blue) and then examined by confocal microscopy. a At 1 day padp, P7-1 and P9-1 antigens accumulated in midgut epithelial cells. Panel I is an enlarged image of the boxed area in panel A showing P7-1 and P9-1 in the epithelial cells. b By 2 days padp, more P7-1 and P9-1 antigens had accumulated in the midgut epithelial cells. c At 4 days padp, P7-1 and P9-1 antigens appeared in the circular muscle and longitudinal muscle of the midgut while (d) some P7-1 and P9-1 antigens were still accumulated in the epithelial cells of the midgut. By 6 days padp, P7-1 and P9-1 antigens had accumulated in (e) the whole digestive system and (f) salivary glands. mg, midgut; me, midgut epithelium; cm, circular muscle; lm, longitudinal muscle; sg, salivary gland; scale bars, 30 μm