L1 and L2 gene polymorphisms in HPV-58 and HPV-33: implications for vaccine design and diagnosis

Table 1 Primers used for the molecular characterization of HPV-33 and HPV-58 L1 and L2

Gene	Primer name	Sequence 5′ to 3′	Primer position	Product size (bp)	Annealing Temperature
33 L1	HPV33 L1 1 F	ATTTGTTCCTATTTCGCCT	5468	921	54 °C
	HPV33 L1 1R	CAGCCCTATTAAAAAAGTGTC	6388
	HPV33 L1 2 F	CAGTACATGCAAATATCCAGATTA	6271	913	54 °C
	HPV33 L1 2R	ACATACACAAAAAACAAACAACA	7183
33 L2	HPV33 L2 1 F	GCACATGGTGGTGTTTTAAC	4116	935	54 °C
	HPV33 L2 1R	AGTCAGGATCAGGAGCAGGT	5050
	HPV33 L2 2 F	AATGTAACATCAAGCACGCC	4834	994	55 °C
	HPV33 L2 2R	TAAACGGACCCTAAAAACCC	5827
58 L1	HPV58 L1 1 F	TCCATTTATTCCTATATCTCCACTA	5515	876	56 °C
	HPV58 L1 1R	GAAAAAAGAACAAACTATCCCCA	6390
	HPV58 L1 2 F	ATGGTAGATACAGGGTTTGGAT	6240	1044	55 °C
	HPV58 L1 2R	AAACAGGAAACTGACAAAGACA	7283
58 L2	HPV58 L2 1 F	GTATACTGGTTATGCACATGGT	4125	847	54 °C
	HPV58 L2 1R	AAAAAAGCAGGGTCAACAAC	4971
	HPV58 L2 2 F	AATGGATACCTTTGTTATTTCTAC	4834	992	54 °C
	HPV58 L2 2R	TACTTTTTTATTGTTATTGGGACT	5825

ISSN: 1743-422X