Fig. 2

Th17 and Treg cells are involved in CVB3-induced myocarditis. Male BALB/c mice (n = 20 per group) were infected with PBS or CVB3 on day 0. The frequencies of CD4⁺IL-17⁺ Th17 cells or CD4⁺CD25⁺Foxp3⁺ Treg cells in mice from spleen on indicated days postinfection were analyzed by Flow cytometry (a and c, left panels). Bar graphs showed the representative percentages of Th17 cells or Treg cells (b and d, right panel). The serum levels of characteristic cytokines IL-17A (e) and IL-10 (f) in CVB3 infection mice were determined by ELISA. Results are presented as the mean ± SEM of three independent experiments. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, compared with PBS group